ABSTRACT
The sensitivity of diploid and heteroploid cell lines for detection of cytotoxicity using the agar diffusion method on cell culture, was tested with ascorbic acid solution of different concentrations. A total of 562 samples of 21 various materials were tested. The heteroploid cell line, RC-IAL, showed in relation to the MRC-5 and HeLa cell lines, greater sensitivity because it showed the presence of cytotoxic effect with the lowest concentration used (10 and 25 micrograms/ml) of ascorbic acid and showed greater diameter of cytotoxic halo in 15 samples and equal diameter in 16 of the 43 positive samples (7.6). Out of 43 positive samples, the MRC-5 line did not show cytotoxicity in 3 sponge samples and 1 of acrylic resin. The PVC (polyvinylchloride) and polyethylene rarely showed positivity, while, the plastic, cotton and acrylic resin demonstrated cytotoxicity in about 5 of samples. We thus suggest the use of the RC-IAL and HeLa cell lines for continuation of this type of analysis at Adolfo Lutz Institute.
Subject(s)
Humans , Animals , Rabbits , Biocompatible Materials/toxicity , Cell Line , Fetus , HeLa Cells , Kidney , Lung , Sensitivity and SpecificityABSTRACT
Em testes de citotoxicidade in vitro, pelo método de difusão em ágar, para materiais de uso médico hospitalar e outros, foram usadas 6 linhagens celulares estabelecidas: AV3, Vero, RC-IAL, NCTC clone 929, RV-IAL, HeLa e cultura primária de embrião de galinha (EG). Nos 20 materiais testados, as células EG demonstraram maior sensibilidade, seguidas das células RC-IAL e HeLa. O efeito citotóxico foi avaliado pela presença e pelas medidas dos diâmetros dos halos difundidos. Devido às dificuldades operacionais com a cultura primária EG recomenda-se, para os testes de citotoxicidade in vitro dos materiais citados, o uso de placas, em duplicata, das células RC-IAL e HeLa (AU).
Subject(s)
Humans , Humans , Cross Infection , Culture TechniquesABSTRACT
A gamaglobulina fornecida por um laboratório nacional experimentada em culturas celulares de linhagem contínua de rim humano conferiu proteção apreciável contra a ação do vírus do sarampo, em doses de 0,1 mg/ml, e proteção total em doses de 0,2 ou 0,3 mg/ml. (AU)
The gamma globulin obtained from the ISA Laboratory was tested on contínuous line of human kidney cells, In experimental condítíons the gamma globulin showed a sígnifleant protectíon against measles vírus, when used in doses of 0,1 mg per ml, and total protection with 0,2 or 0,3 mg per ml. (AU)